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KMID : 0903619960370030428
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Journal of the Korean Society for Horticultural Science
1996 Volume.37 No. 3 p.428 ~ p.434
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Characteristics of Hybrid Plants Obtained by Zygotic Embryogenesis from Immature Ovule Crossed Between Korean Native Citrus junos and Poncirus trifoliata
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Abstract
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Immature ovules of Korean native Citrus junos Sieb. ex Tanaka (yuzu, acid citrus) crossed with Poncirus trifoliata Raf. (trifoliate orange) were cultured in vitro for the improvement of tree vigor, nonbearing periods, and fruit quality characters. F₁ and BC₁ plants were successfully regenerated from zygotic embryos of in vitro cultured ovules excised on the 4th to the 5th week after pollination. Zygotic embryos were observed to appear in the micropylar region of Citrus junos ovule at excision time. Tree vigor of selected F₁ plants was very low, and the fruit quality in general was undesirable because of high acidity and rough skin. BC₁ hybrid plants also showed very low vigor and were very precocious with fruits of low acid content and slightly smooth skin. Fruit size, however, was smaller than that of mother plants. Further improvement of fruit quality of BC₁ hybrid plant was considered to be needed for future marketability. Isozyme analysis for the identification of selected F₁ and BC₁ plant was analyzed by gel electrophoresis for peroxidase (PRX) and glutamate oxaloacetate transaminase (GOT) banding patterns. The selected hybrids derived from in vitro culture of the micropylar part of immature ovules were identified as zygotic seedlings.
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